Composite

Part:BBa_I728978:Design

Designed by: Bernice Huang   Group: iGEM07_MIT   (2007-10-23)


Promoter+RBS+CPX(Circularly-Permuted OmpX for Surface Display of Polypeptides)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

We decided to use F2620 as a promoter because we weren't sure how many displayed copies of our engineered CPX could be handled by e. coli and F2620 allowed us to test multiple levels of transcription and translation of CPX to find the most effective rates.


Source

CPX sequence comes from Rice et. al "Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands", while the polystyrene-binding insert sequence orinated from Kenan et. al "Peptide-PEG Amphiphiles as Cytophobic Coatings for Mammalian and Bacterial Cells"

References